Characterization of probiotic Escherichia coli isolates using a novel pangenome microarray

ABSTRACT: Background: Based on 32 Escherichia coli and Shigella genome sequences, we have developed an E. coli pan-genome microarray. Publicly available genomes were annotated in a consistent manor to define all currently known genes potentially present in the species. The chip design was evaluated by hybridization of DNA from two sequenced E. coli strains, K-12 MG1655 (a commensal) and O157:H7 EDL933 (an enterotoxigenic E. coli). A dual channel and single channel analysis approach was compared for the comparative genomic hybridization experiments. Moreover, the microarray was used to characterize four unsequenced probiotic E. coli strains, currently marketed for beneficial effects on the human gut flora. Results: Based on the genomes included in this study, we were able to group together 2,041 gen

ORGANISM(S): Escherichia coli

SUBMITTER: Peter Hallin 

PROVIDER: E-GEOD-8595 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress