Transcription profiling of E. coli BW25113 yncC vs wild type biofilm cells in LB 15h 37C and MG1655 yncC vs wild type colony cells in LB plates 15h 37C

ABSTRACT: E. coli K-12 BW25113 mutant strain yncC expression in biofilm cells relative to E. coli wild-type strain expression in biofilm cells. All samples were cultured in LB with glasswool at 37C for 15 hours and E. coli K-12 MG1655 mutant yncC colony cells vs wild type colony cells in LB plates 15h 37C. Quorum-sensing signal autoinducer 2 (AI-2) stimulates Escherichia coli biofilm formation through the motility regulator MqsR that induces expression of the putative transcription factor encoded by yncC. Here we show YncC increases biofilm formation by decreasing mucoidy (corroborated by decreased exopolysaccharide production and increased sensitivity to bacteriophage P1 infection). Differential gene expression and gel shift assays demonstrated that YncC is a repressor of the predicted periplasmic

ORGANISM(S): Escherichia coli

SUBMITTER: Xue-Song Zhang 

PROVIDER: E-GEOD-8706 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress