Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse Nrf2 knockout caused by ROS-mediated insulin/IGF-1 resistance


ABSTRACT: The liver is frequently challenged by surgery-induced metabolic overload, viruses, or toxins, which induce the formation of reactive oxygen species. To determine the effect of oxidative stress on liver regeneration and to identify the underlying signalling pathways, we studied liver repair in mice lacking the Nrf2 transcription factor. In these animals, expression of several cytoprotective enzymes was reduced in hepatocytes, resulting in oxidative stress. As a consequence, tissue damage was aggravated, and liver regeneration after partial hepatectomy was delayed. Using in vitro and in vivo studies we identified oxidative stress-induced insulin/insulin-like growth factor resistance as the underlying mechanism. This deficiency impaired the activation of p38 mitogen-activated kinase, Akt kinase, and downstream targets after hepatectomy, resulting in enhanced death and delayed proliferation of hepatocytes. Our results reveal novel roles of Nrf2 in the regulation of growth factor signalling and in tissue repair. In addition, they provide new insight into the mechanisms underlying oxidative stress-induced defects in liver regeneration and thus offer new avenues to improve regeneration in patients with acute or chronic liver damage. Experiment Overall Design: Livers from Nrf2 k.o. and wt mice; 3 hybridizations per genoype: RNA samples were pooled from 3 individual animals

ORGANISM(S): Mus musculus

SUBMITTER: Stefan Zoller 

PROVIDER: E-GEOD-8969 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impaired liver regeneration in Nrf2 knockout mice: role of ROS-mediated insulin/IGF-1 resistance.

Beyer Tobias A TA   Xu Weihua W   Teupser Daniel D   auf dem Keller Ulrich U   Bugnon Philippe P   Hildt Eberhard E   Thiery Joachim J   Kan Yuet Wai YW   Werner Sabine S  

The EMBO journal 20071206 1


The liver is frequently challenged by surgery-induced metabolic overload, viruses or toxins, which induce the formation of reactive oxygen species. To determine the effect of oxidative stress on liver regeneration and to identify the underlying signaling pathways, we studied liver repair in mice lacking the Nrf2 transcription factor. In these animals, expression of several cytoprotective enzymes was reduced in hepatocytes, resulting in oxidative stress. After partial hepatectomy, liver regenerat  ...[more]

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