Transcription profiling of mouse embryoid bodies with HOXB4 induced or not induced to identify its target genes

ABSTRACT: To unravel the molecular mechanism by which HOXB4 promotes the expansion of early hematopoietic progenitors within differentiating ES cells, we analzed the gene expression profiles of embryoid bodies (EBs) in which transcription of HOXB4 had been induced or not induced. A substantial number of the identified HOXB4 target genes are involved in signaling pathways important for controlling self-renewal, maintenance and differentiation of stem cells. Furthermore, we demonstrate that HOXB4 activity and FGF-signaling are intertwined. HOXB4-mediated expansion of ES cell-derived early progenitors was enhanced by specific and complete inhibition of FGF-receptors. In contrast, the expanding activity of HOXB4 on hematopoietic progenitors in day4-6 embryoid bodies was blunted in the presence of basic

ORGANISM(S): Mus musculus

SUBMITTER: Moises Mallo 

PROVIDER: E-GEOD-9044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress