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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human primary nasal epithelium exposed to house dust mite extract shows activated expression in allergics

ABSTRACT: Response to allergen was studied in epithelial cells derived from allergic pantients and from healthy controls. Cells were cultured after isolation from a nasal biopsy. Cells were exposed to Housed dust mite or vessel (saline); Microarray data was analysed using bioinformatics and biostaistics. We conclude that a marked difference in basal expression and in response to hous dust mite exists Experiment Overall Design: We used 5 monotypic house dust mite allergic patients and 5 non-allergic healthy controls, the cells obtained from the biopsies were cultured in two wells, for two different conditions.

ORGANISM(S): Homo sapiens

SUBMITTER: Aram Ben Vroling

PROVIDER: E-GEOD-9150 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Primary nasal epithelium exposed to house dust mite extract shows activated expression in allergic individuals.

Vroling Aram B AB Jonker Martijs J MJ Luiten Silvia S Breit Timo M TM Fokkens Wytske J WJ van Drunen Cornelis M CM

American journal of respiratory cell and molecular biology 20070927 3

Nasal epithelial cells form the outermost protective layer against environmental factors. However, this defense is not just physical; it has been shown that epithelial cells respond by the production of inflammatory mediators that may affect local immune responses. In this research we set out to characterize potential differences between the responses of nasal epithelium from healthy and allergic individuals to house dust mite (HDM) allergen. These differences will help us to define local mechan ...[more]

PMID: 17901406

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Primary nasal epithelium exposed to house dust mite extract shows activated expression in allergics

Project description:Response to allergen was studied in epithelial cells derived from allergic pantients and from healthy controls. Cells were cultured after isolation from a nasal biopsy. Cells were exposed to Housed dust mite or vessel (saline) Microarray data was analysed using bioinformatics and biostaistics. We conclude that a marked difference in basal expression and in response to hous dust mite exists Keywords: cellular response to allergen

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