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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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SiRNA profiling of human LNCaP cells overexpressing/knockdown of HOXC6

ABSTRACT: The transcription factor HOXC6 is upregulated in human prostate cancer. SiRNA knockdown of HOXC6 induces apoptosis in LNCaP cells while upregulation rescued LNCaP cells from siRNA-induced apoptosis. We used microarrays to identify the genes whose expression underly the anti-apoptotic and proliferative effects of HOXC6 in LNCaP cells. Experiment Overall Design: LNCaP cells were transiently transfected with a cocktail of 5 siRNAs to HOXC6 to a final concentration of 100nM, with a HOXC6 gene expression vector, or control siRNA and vector. Cells were collected a 48hours post-transfection

ORGANISM(S): Homo sapiens

SUBMITTER: Colleen McCabe

PROVIDER: E-GEOD-9182 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from overexpression and knockdown of HOXC6 in LNCaP cells

Project description:The transcription factor HOXC6 is upregulated in human prostate cancer. SiRNA knockdown of HOXC6 induces apoptosis in LNCaP cells while upregulation rescued LNCaP cells from siRNA-induced apoptosis. We used microarrays to identify the genes whose expression underly the anti-apoptotic and proliferative effects of HOXC6 in LNCaP cells. Keywords: transient overexpression and knockdown

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