Gene expression profiling in heterophils with Salmonella enteritidis challenge using a chicken 44K Agilent microarray

ABSTRACT: To determine the transcriptional response to Salmonella enterica serovar Enteritidis (SE) infection, a newly developed chicken 44K Agilent array was used to analyze total RNA of heterophils from SE-resistant (line A) and SE–susceptible chickens (line B), treated with in vitro infection of SE (I) or non SE medium (N) for 1 hr. A dual-color balanced design was used to provide a direct comparison between SE-infected and non-infected groups (AI/AN, BI/BN) and between line A and line B (AN/BN, AI/BI). Data retrieved from 426 immunologically important genes were used for functional analysis. The results indicated that: In the comparisons of SE infection with non-infection, 39 genes were found differentially expressed (P < 0.05 with at least 2-fold) after Salmonella infection, while 21 genes we

ORGANISM(S): Gallus gallus

SUBMITTER: Huaijun Zhou 

PROVIDER: E-GEOD-9416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress