Transcription profiling of mesenchymal stem cells to produce a signature for MSC

ABSTRACT: Background. The in vivo distribution status and molecular signature of bone marrow mesenchymal stem cells (MSC) remain unknown, although ex vivo expanded MSC have been used in numerous studies. Methods/Results. To address these issues, we identified human MSC-characteristic genes, including 9 transcription factor genes - using DNA microarray analysis: Most (75 %) of the MSC-characteristic genes were down-regulated by knockdown of the transcription factors. Furthermore, we used an antibody set for the MSC-characteristic molecules in immunohistochemistry, which revealed that, in embryonic/neonatal mice, elongated MSC were abundant near the endosteum and in the interior of bone marrow, perichondrium and periosteum, whereas in adult mice round/compact MSC were found only in the interior of bon

ORGANISM(S): Homo sapiens

SUBMITTER: Masakazu SHIMIZU 

PROVIDER: E-GEOD-9451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress