Identification of Signature Molecule-Marked Native Mesenchymal Stem Cells
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ABSTRACT: Background. The in vivo distribution status and molecular signature of bone marrow mesenchymal stem cells (MSC) remain unknown, although ex vivo expanded MSC have been used in numerous studies. Methods/Results. To address these issues, we identified human MSC-characteristic genes, including 9 transcription factor genes - using DNA microarray analysis: Most (75 %) of the MSC-characteristic genes were down-regulated by knockdown of the transcription factors. Furthermore, we used an antibody set for the MSC-characteristic molecules in immunohistochemistry, which revealed that, in embryonic/neonatal mice, elongated MSC were abundant near the endosteum and in the interior of bone marrow, perichondrium and periosteum, whereas in adult mice round/compact MSC were found only in the interior of bone marrow: No MSC-like cells were found in any other skeletal or non-skeletal tissues using this antibody set. Conclusion. The identification of native MSC in developing and adult skeletal tissues should be useful in basic and clinical studies on MSC. Keywords: cell type comparison
ORGANISM(S): Homo sapiens
PROVIDER: GSE9451 | GEO | 2008/10/28
SECONDARY ACCESSION(S): PRJNA103211
REPOSITORIES: GEO
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