Effect of short- and long-term morphine treatment on gene expression in the hypothalamus and pituitary

ABSTRACT: Addictive drugs including opioids activate signal transduction pathways that regulate gene expression in the brain. However, changes in CNS gene expression following morphine exposure are poorly understood. We studied the effect of short- and long-term morphine treatment on gene expression in the hypothalamus and pituitary using genome-wide DNA microarray and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analyses. In the hypothalamus, we found that short-term morphine administration up-regulated (at least 2-fold) 39 genes and down-regulated six genes. Long-term morphine administration up-regulated 35 genes and down-regulated 51 hypothalamic genes. In the pituitary, we found that short-term morphine administration up-regulated (at least 2-fold) 110 genes and down-regula

ORGANISM(S): Mus musculus

SUBMITTER: Adrian Anghel 

PROVIDER: E-GEOD-9525 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress