Microarray-based global mapping of integration sites of retrotransposon, intracisternal A-particle, in mouse genome

ABSTRACT: Mammalian genomes have evolutionary harbored numerous mobile elements, a part of which are still active and evoke genomic instability. Their movement and positional diversity occasionally result in phenotypic changes and variation by causing altered expression and disruption of neighboring genes in the host genome. Here, we describe a novel microarray-based method by which dispersed genomic locations of a type of retrotransposons in mammalian genome can be identified at a time. By this method, we mapped the DNA elements for a mouse retrotransposon, intracisternal A-particle (IAP), within genomes of C3H/He and C57BL/6J inbred mouse strains; consequently we detected hundreds of probable IAP cDNA-integrating genomic regions. Among 147 putative insertions located nearby the promoter region

ORGANISM(S): Mus musculus

SUBMITTER: Takashi Takabatake 

PROVIDER: E-GEOD-9572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress