Transcription profiling of S. cerevisiae TPP 2-oxo acid decarboxylases

ABSTRACT: The irreversible decarboxylation step, which commits 2-oxo acids to the Ehrlich pathway, was initially attributed to pyruvate decarboxylase. However, the yeast genome was shown to harbour no fewer than 5 genes that show sequence similarity with thiamine-diphosphate dependent decarboxylase genes. Three of these (PDC1, PDC5 and PDC6) encode pyruvate decarboxylases { while ARO10 and THI3 represent alternative candidates for Ehrlich-pathway decarboxylases. Transcriptome analysis and decarboxylase activity measurements on an S. cerevisiae aro10 strain, a double aro10 thi3 deletion strain and a quadruple pdc1,5,6,aro10 mutant strains grown in carbon–limited chemostat with phenylalanine as nitrogen source indicated that:; i) PDC5 is strongly upregulated in an aro10 background (Fig. 2) and

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-9590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress