Activation by CcpA-FDP of two key determinants of pneumococcal virulence, capsule production and choline utilization.

ABSTRACT: The kinetic behaviour of S. pneumoniae during vaccine production was studied using a dynamic systemic approach. Quantification of key intracellular glycolytic metabolites coupled to global transcriptomic analysis led to an improved knowledge of pneumococcal physiology. In controlled growth conditions, a direct correlation between the accumulation of key glycolytic intermediates and the expression of capsular polysaccharide genes (cps operon encoding the main pneumococcal antigen) was established. Interestingly, the same correlation was confirmed for the genes involved in the assimilation and the modification of choline, an indispensable nutritional requirement for both growth and virulence of S. pneumoniae. Such a correlation suggests a direct or indirect control of the expression of these

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Nic Lindley 

PROVIDER: E-GEOD-9670 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress