Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Multiple functions for Eya3 in mammalian organism

ABSTRACT: The important function of Eya (eyes absent) in eye development of D.melanogaster is well known, the function of its mammalian orthologs Eya1-4 is only partially understood. Yet, no phenotype for Eya2 and Eya3 in humans or mouse have been described. In our study we analysed new Eya-deficient mouse model generated by insertional mutagenesis. Expression analysis of Eya3 by in-situ hybridizations and M-NM-2-Gal-staining revealed a broad expression of the gene throughout development, e.g. in brain, eyes, branchial arches, somites and limbs. This diverse expression pattern suggests pleiotropic effects of Eya3 in mammalian organisms. In contrast to D. melanogaster, a close examination of the eye by various methods did not show any obvious ocular defects in mutant animals. A broad phenotypical characterization of homozygous mutants displayed decreased body weight and body length. Additionally, behavioral changes as a educed forward locomotor activity and altered exploratory behaviour were identified. A significant reduction of forelimb grip strength and an impairment of cardiac processes in Eya3-/- mice suggest a role for Eya3 in muscle development and heart function. Our results implicate that Eya3 plays various roles outside the eye with effects particularly on heart and muscle function in the mouse. Keywords: Eya3-deficient mice, cDNA microarrays, brain, heart, muscle analysis of 4 Eya3 mutant mice versus a pool of 5 wildtype animals; brain, heart, and muscle; 8 hybridisations for each tissue: two technical replicates for each mutant animal including one dye-swap experiments

ORGANISM(S): Mus musculus

SUBMITTER: Marion Horsch

PROVIDER: E-GEOD-9999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pleiotropic effects in Eya3 knockout mice.

Söker Torben T Dalke Claudia C Puk Oliver O Floss Thomas T Becker Lore L Bolle Ines I Favor Jack J Hans Wolfgang W Hölter Sabine M SM Horsch Marion M Kallnik Magdalena M Kling Eva E Moerth Corinna C Schrewe Anja A Stigloher Christian C Topp Stefanie S Gailus-Durner Valerie V Naton Beatrix B Beckers Johannes J Fuchs Helmut H Ivandic Boris B Klopstock Thomas T Schulz Holger H Wolf Eckhard E Wurst Wolfgang W Bally-Cuif Laure L de Angelis Martin Hrabé MH Graw Jochen J

BMC developmental biology 20081222

<h4>Background</h4>In Drosophila, mutations in the gene eyes absent (eya) lead to severe defects in eye development. The functions of its mammalian orthologs Eya1-4 are only partially understood and no mouse model exists for Eya3. Therefore, we characterized the phenotype of a new Eya3 knockout mouse mutant.<h4>Results</h4>Expression analysis of Eya3 by in-situ hybridizations and beta-Gal-staining of Eya3 mutant mice revealed abundant expression of the gene throughout development, e.g. in brain, ...[more]

PMID: 19102749

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Dataset Information

Multiple functions for Eya3 in mammalian organism

Publications

Pleiotropic effects in Eya3 knockout mice.

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