Transcription profiling of Geobacter sulfurreducens wild type and pilR mutants with acetate as the electron donor and ferric citrate as the electron acceptor

ABSTRACT: G. sulfurreducens wild type and pilR mutant (GSU1495) were grown in chemostats for RNA extraction used for microarray analysis and qRT-PCR. The electron donor (acetate 5mM) was limiting at a dilution rate of 0.05 h-1 and ferric citrate(55mM) was used as the electron acceptor at 30C, as previously described (Esteve-Nunez et al., 2005). Analysis of acetate, Fe(II) and protein were performed as previously described (Esteve-Nunez et al., 2005). Disruption of the pilR gene (GSU1495) was made in G. sulfurreducens strain DL1 (ATCC 51573) by the recombinant PCR and single-step recombination method (Murphy et al., 2000), essentially as described (Lloyd et al., 2003). To disrupt the pilR gene a 2.25 kb DNA fragment was constructed by PCR in which 0.39 kb of the pilR coding sequence (codons 192 - 323

ORGANISM(S): Geobacter sulfurreducens

SUBMITTER: Barbara Methe 

PROVIDER: E-JCVI-1 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress