Transcription profiling by array of hMADS cells transfected with miR-26a and induced to differentiate into adipocytes
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ABSTRACT: Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA). Two days later (=d0), adipocyte differentiation was induced. At d9 after start of differentiation, cells were harvested to identify mRNAs that are down- and upregulated by miR-26a versus miR-C.
Project description:Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA) and cells were harvested two days later (=d0) to identify mRNAs that are downregulated by miR-26a versus miR-C, as such mRNAs could be direct miR-26a targets.
Project description:Comparison of the genetic profile of hBM-MSC differentiated in vitro into osteoblasts with in vivo developed osteoblasts derived from hip bone.
Project description:hBM-MSC were expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability;õA analysis were performed p2/p5 (n=10)and p5/p10 (n=5) to examine if the genetic background of hBM-MSC is changeing during their high-grade expansion;
Project description:hBM-MSC were expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability;õA analysis were performed p2/p5 (n=10)and p5/p10 (n=5) to examine if the genetic background of hBM-MSC is changeing during their high-grade expansion;
Project description:Differentiation of bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction; reference d-1 (one day before induction; preconfluent 70-80%)
Project description:Experiments were designed to compare white adipose tissue (WAT) or brown adipose tissue (BAT) -in male and female mice- between Bscl2 knock-out mice and their wild-type control mice. RNA was pooled to obtain 2x 8µg per tissue source and subjected to dye-swap hybridization.
Project description:This experiment was designed to identify C/ebp alpha target genes during the process of adipogenesis by means of comparison of differentiating (wild-type) cells to a perturbation model (transgenic cells). For this, gene expression profiling during adipogenesis of mouse embryonic fibroblasts (MEFs) prepared from wild-type and transgenic mice was performed. The transgene encodes for a dominant-negative A-CEBP protein that abolishes C/EBP DNA-binding activity. Five timepoints were measured for both transgenic and wild-type MEFs during adipogenesis (0h, 10h, 20h, d3 , d8). Every timepoint was hybridized against pre-confluent cells as reference.
Project description:The aim of the project is to identify the late (24h) host response related to Gram positive (S. pyogenes) or Gram negative (E. coli) induced sepsis in a controlled sepsis model 24 hours a. Therefore a well defined baboon sepsis model, established at the Ludwig Boltzmann Institute for experimental and clinical traumatology of Vienna, is used. The overall goal of the project is to define differentially expressed or processed genes which give rise to diagnostic and therapeutic targets and consequently better monitoring and earlier onset of therapy.
Project description:The aim of the project is to identify the host response related to Gram positive (S. pyogenes) or Gram negative (E. coli) induced sepsis in a controlled sepsis model. Therefore a well defined baboon sepsis model, established at the Ludwig Boltzmann Institute for experimental and clinical traumatology of Vienna, is used. The overall goal of the project is to define differentially expressed or processed genes which give rise to diagnostic and therapeutic targets and consequently better monitoring and earlier onset of therapy.