Transcription profiling of Daphnia magna treated with: cadmium, pH, lufenuron, calcium limitation and ibuprofen for 24 performed in replicate to assess their effect on stress reponse and growth

ABSTRACT: Microarray Construction The D. magna microarray was constructed using DNA fragments from three sources: i) Stress-specific cDNAs were generated using Selective Subtractive Hybridisation (SSH) on organisms exposed to the stressors shown in Table 1. SSH was performed with forward and reverse subtraction using a PCR-Select cDNA Subtraction Kit (Clontech Laboratories Inc, USA). cDNA was prepared from mRNA extracted from batches of 100 D. magna, 48 h old, using a Straight AM-CM-"M-BM-,s mRNA Isolation Kit System (Novagen Inc., UK) (2,029 clones). ii) ESTs obtained from a cDNA library from unexposed mixed aged organisms obtained from a 3-4 week old culture (Watanabe et al. 2005) (10,272 clones). iii) cDNAs isolated following an SSH, as above, between 15 adults carrying eggs and 75 juveniles

ORGANISM(S): Daphnia magna

SUBMITTER: unknown unknown 

PROVIDER: E-MAXD-20 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress