Transcription profiling of dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild type (WT) and six Mdm4-null mouse embryos (KO)

ABSTRACT: Total RNA was isolated from the dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild-type (WT) and six Mdm4-null embryos (KO). Equal quantities of total RNA from single samples were used to obtain four RNA pools (WT-Neur, WT-N.Neur, KO-Neur, KO-N.Neur), each representing one of the experimental samples under analysis. Biotin labeled cRNA targets were obtained starting from 10ug of total RNA derived from the RNA pools. cDNA synthesis was performed with GIBCO SuperScript Custom cDNA Synthesis Kit, and biotin-labeled antisense RNA was transcribed in vitro using Ambion's In Vitro Transcription System, and including Bio-16-UTP and Bio-11-CTP (Enzo) in the reaction. Each labeled target was hybridized to two copies of the the complete Affymetrix MG-U174v2 chip set.

ORGANISM(S): Mus musculus

SUBMITTER: Eric Bellefroid 

PROVIDER: E-MEXP-155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress