Transcription profiling of three human colorectal adenocarcinoma cells lines two which are mismatch repair deficient to characterize the post-transcriptional profile
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ABSTRACT: characterize the post-transcriptional profile of mismatch repair-deficient colon cancer
Project description:The representative cell line, HT29, culture in hypoxic and normoxic conditions were used to perform the miRNA microarray. The chip assay and data analysis were entrusted to KangChen Bio-tech, Inc. (Shanghai, China) and all the miRNAs with more than 2-fold variation in different samples were identified. Detailed methods were performed as previously described24 using the miRCURY Hy3 labeling kit and the microRNA array software (Exiqon, Denmark).
Project description:Multiple genome-wide microarrays have been used to analyse the transcriptomes of immunomagnetically separated normal human luminal epithelial and myoepithelial cells, as well as primary malignant breast epithelial cells.
Project description:Investigation of the alterations in the gene expression pattern of peripheral blood monocytes after constitutive adhesion to naive human umbilical vein endothelial cells
Project description:The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays
Project description:Arabidopsis thaliana wildtype (Col-0) was compared with the ntrc mutant under different photoperiods (short day: 8h/16h; long day 16h/8h light/dark) and different ages (10d and 28/21d).
Project description:Mice were obtained from in house breeding of C57BL/6J and C57BL/6J-Chr 1A/Na breeding pairs (Jackson Laboratories, USA). To produce F1 hybrids, C57BL/6J females were mated with C57BL/6J-Chr 1A/Na males. The F1 hybrids were intercrossed, producing 82 F2 progeny (41 males and 41 females). Microarray analysis was performed on six pairs of affected and non-affected male animals from the F2 progeny selected on the basis of their motor activity levels (average daily levels of distance moved over a 3 days recording: 768±74 cm/hr (affected) versus 1765±175 cm/hr (non-affected)(p<0.0001).