Transcription profiling of Arabidopsis seeds from wild type and srk2d srk2e srk2i mutant plants
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ABSTRACT: Microarray experiments of the srk2d srk2e srk2i seeds were performed by analyzing the samples obtained from two independent growth conditions (both for wild-type and mutant).For the analysis of gene expression profiles in seeds of srk2d srk2e srk2i, RNA from Col (CS60000) seeds was used.
Project description:Microarray experiments of the abi5 seeds were performed by analyzing the samples obtained from two different alleles (both for wild-type and mutant). For the analysis of gene expression profiles in abi5-1 seeds, RNA from Ws seeds was used. For the analysis of gene expression profiles in abi5-7 seeds, RNA from Col seeds was used.
Project description:Microarray experiments of the abi3 seeds were performed by analyzing the samples obtained from two different alleles (both for wild-type and mutant).For the analysis of gene expression profiles in abi3-1 seeds, RNA from Ler seeds was used. For the analysis of gene expression profiles in abi3-6 seeds, RNA from Col seeds was used.
Project description:Arabidopsis thaliana (L.) Heynh. ecotype Columbia was used in this study. Seeds were sterilized and stratified at 4 C for 2 days in the dark, sown, and grown on germination media (GM) agar plates in a growth chamber with 16 hours of fluorescent light at 40M-110 ?mol m-2 sec-1, 22 C, and 70% relative humidity.<br><br> The T-DNA insertion line for GRF7 was obtained from the ABRC (Stock No. CS878963;? Sessions et al., 2002). The T-DNA insertion was confirmed by amplifying the LB-flanking region in genomic DNA of individual candidate lines.
Project description:Gene expression profiles in transgenic rice plants that overexpressed OsNAC5, driven by the maize ubiquitin promoter (UBIpro::OsNAC5).
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 M-1 10 ?mol photons/m2/s) at 28M-0C (day) and 25M-0C (night). For cold treatment, two-week-old plants were transferred from 28M-0C to 10M-0C and incubated for 1 day.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 ± 10 ?mol photons/m2/s) at 28°C (day) and 25°C (night). For dehydration treatment, two-week-old plants were incubated for 3 days without watering. The soil moisture content was 15.6% on day 3 of dehydration.
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with or without 50 µM ABA for 6 h.
Project description:Effect of single or double knock-out mutation of subclass III SnRK2 protein kinases in Arabidopsis after 6 h of treatment with dehydration stress.
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with stress treatment of dehydration for 6 h.