Chromatin immunoprecipitation of wild type msc1D and swr1D fission yeast to determine genome-wide distribution of H2A.Z

ABSTRACT: Eukaryotic genomes are repetitively packaged into chromatin by nucleosomes, however they are regulated by the differences between nucleosomes, which establish various chromatin states. Replication-independent histone exchange could potentially perturb chromatin status if histone exchange chaperones, such as Swr1C, loaded histone variants into wrong sites. Here we use ChIP-chip analysis of H2A.Z-myc to show that in S.pombe, like S.cerevisiae, Swr1C is required for loading H2A.Z into specific sites, including the promoters of lowly expressed genes. However S.pombe Swr1C has an extra subunit, Msc1, which is a JumonjiC-domain protein of the Lid/Jarid1 family. The absence of Msc1 does not disrupt the S.pombe Swr1C or H2A.Z distribution in euchromatin. However in the absence of either Msc1 or Sw

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Luke Buchanan 

PROVIDER: E-MEXP-1986 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress