Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Overexpression of Mitochondrial Aconitase in HT29 Colon Cancer Cells Attenuates beta-Catenin Signaling Pathway via Metabolic Changes of NADPH and ROS

ABSTRACT: Our previous proteomics analysis suggested down-regulation of mitochondrial aconitase (ACO2) plays an important role in colorectal cancer. To evaluate the effect of mitochondrial aconitase overexpression on the metabolic and signaling pathway changes in colon cancer cell line HT29, we generated two ACO2 overexpressing cell lines #C and #UD and the mock-transfected vector control #VE. HT29 colon cancer cells overexpressing ACO2 exhibited lower rate of cell proliferation in vitro and reduced tumor growth potential in nude mice. In order to understand the signaling pathway changes, cDNA microarray analysis using GeneChips from Affymetrix were carried out.

ORGANISM(S): Homo sapiens

SUBMITTER: Xuezhi Bi

PROVIDER: E-MEXP-1996 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

miRNA profiling of human colon cell line HT29 under normoxic and hypoxic treatment.

Project description:The representative cell line, HT29, culture in hypoxic and normoxic conditions were used to perform the miRNA microarray. The chip assay and data analysis were entrusted to KangChen Bio-tech, Inc. (Shanghai, China) and all the miRNAs with more than 2-fold variation in different samples were identified. Detailed methods were performed as previously described24 using the miRCURY Hy3 labeling kit and the microRNA array software (Exiqon, Denmark).

2010-01-06 | E-MEXP-2507 | biostudies-arrayexpress

Identification of common human host genes involved in pathogenesis of different rota virus strains-an attempt to recognize probable antiviral targets

Project description:In this study, we aim to identify common human host genes involved in pathogenesis of different rota virus strains as an attempt to recognize probable antiviral targets. We have compared the host gene regulation after infection of human intestinal cell line (HT29) with three different wild type RV strains i.e. SA11 (simian, G3, P2), A5-13 (bovine, G8, P1) and Wa (human, G1, P8). HT29 cells mock infected or infected with three rota virus strains (SA11, A5-13, Wa). At 5hpi total RNA was extracted and microarray was done using Affymetrix protocol.

2013-06-30 | E-MEXP-3670 | biostudies-arrayexpress

The RALA pathway can maintain the proliferation of KRAS- and BRAF-mutated cancer cells

Project description:By silencing of RALA, a downstream member of the RAS signal transduction pathway, we aimed to determine whether genes downstream of a mutated KRAS (codon 12 or 13) or a mutated BRAF can have significant functions in colorectal cancer carcinogenesis. RALA was silenced in three colorectal cancer cell lines (SW480, HCT116 and HT29). Effects were normalized to mock-transfected cells and the effects of scramble siRNA were excluded. SW480, HCT116 and HT29 cell lines were treated with the PI3K inhibitor LY294002 or DMSO.

2015-06-20 | E-GEOD-39857 | biostudies-arrayexpress

Role of miR-146a in colon cancer cells

Project description:miRNAs exert various biological functions by targeting different cellular targets. Studying miR-146a functions in colon cancer cells helps to understand colorectal cancer (CRC) malignancy and progression. HT29 miR-146a-expressing cells and empty control cells were established for mining the gene expression profiles upon miR-146a expression.

2013-11-20 | E-GEOD-37596 | biostudies-arrayexpress

Colon cancer shperes are sensitive to Fenretinide

Project description:Fenretinide has shown its antitumor activity in many tumor types with low cytotoxicity to normal cells. Recently, we have shown that fenretinide could eradicate chronic myeloid leukemia stem/progenitor cells and spheres from ovarian cancer. In this study, we investigate whether fenretinide could selectively target sphere cells of colon cancer. Using high-throughtput microarray system, we identified GO terms and pathway signatures enriched in fenretinide treated HT29 cells and HT29 derived sphere cells. Colon cancer cells HT29 were cultured in sphere formation conditions, HT29 cells and HT29 derived sphere cells were treated with fenretinide, and total RNA from those spheres and corresponding adhered cell was hybridized on Affymetrix U133 plus 2.0 genechip.

2016-04-03 | E-GEOD-66983 | biostudies-arrayexpress

Transcription profiling of human colorectal carcinoma cell lines HCT116 and HT29 treated with 5-ASA

Project description:Transcription profiling of human colorectal carcinoma cell lines HCT116 and HT29 treated with 5-ASA

2009-11-16 | E-TABM-768 | biostudies-arrayexpress

The antidepressant fluoxetine elicits anticancer effects by impairing aerobic glycolysis in colon cancer cells and xenografts.

Project description:Colon cancer is one of the most common tumors worldwide. Recent reports showed that patients treated with the antidepressant fluoxetine had reduced colon cancer risk, with effects similar to the chemotherapeutic 5-fluoro-uracil. Here, we examined the effects of fluoxetine and 5-fluoro-uracil on gene expression of HT29 colon cancer cell xenografts. HT29 xenografts in NOD/SCID mice were treated with vehicle (physiological solution), fluoxetine (30mg/kg/day), or 5-Fluoro-uracil (50 mg/kg/day).

2015-06-09 | E-GEOD-51904 | biostudies-arrayexpress

Diverse effect of WWOX overexpression in HT29 colon cancer cell line.

Project description:The WWOX gene is a tumor suppressor probably involved in regulation of cell cycle and apoptosis and downregulated in variety of cancer types.However, its role in colon cancerogenesis is unknown. The aim of this study was to characterize how WWOX may be involved in colon cancerogenesis or cancer progression, how it influences the basic cancer cell features and modifies cell expression profile.Our observations suggest that in HT29 colon cancer cell line increased expression of WWOX may result in transition of cancer cells into more normal- like colon epithelium phenotype, on the other hand in SW480 WWOX revealed the well-known tumour suppressor properties. However, as the colon cancer is very heterogeneous disease, obtained discrepancies may reflect the known differences between cell lines and cancerogenesis pathway, which they undergone. HT29 colon cancer cells were stably transfected with WWOX cDNA. HT29 cells transfected with an empty vector served as a control. Total mRNA was isolated to look for gene-expression differences induced by the WWOX overexpression.

2014-01-23 | E-GEOD-54298 | biostudies-arrayexpress

Transcription profiling of human short-time cultured primary cells derived from colorectal adenocarcinoma

Project description:Time course comparison to tissue origin and with control cell line HT29 derived from colorectal adenocarcinoma. Status of expression pattern is different in adenocarcinoma of each patient. Human tumor cells extensively changes their gene- and protein expression patterns during their cultivation, clonal selection and expansion, thereby loosing many of the characteristics of their primary origin. In this study we analyzed if these expression changes could be circumvented by using short-term primary cell culture models derived from colorectal cancer patients. We compared several primary cells from tumor tissues using a standardized protocol which yielded similar cell populations. For monitoring the gene expression changes induced by cell preparation and cultivation we collected the tissues immediately after resection and isolated cells before seeding, and after 24 and 72 hours of cultivation from each patient. Experiment Overall Design: Reference tissue, viable primary cells, and cells of cell line HT29 were selected at indicated times, total RNA's were preparated and amplified to biotinylated cRNA for microarray analysis using high-density HG-U133plus 2.0 GeneChipsÂ® from Affymetrix.

2008-10-26 | E-GEOD-13059 | biostudies-arrayexpress

Antitumor lectin from Sclerotium rolfsii (SRL) induces apoptosis in human colon cancer HT29 cells by activation of multiple signaling pathways; A microarray analysis

Project description:TF antigen specific Sclerotium rolfsii lectin (SRL) induces apoptosis in human colon cancer HT29 cells and has tumour suppressing effect in vivo as reported earlier. In this study, we investigated the signaling pathways to identify the signature genes that lead to apoptosis of HT29 cells by mRNA and miRNA microarray analysis. HT29 cells were treated with SRL (20 ug/ml) for 2, 4, 8 12, 24 and 48h and gene expression microarray analysis was performed using SurePrint G3 Human Gene Expression Microarray kit. Several hundred entities were differentially regulated with a fold change value of greater than or equal to 2 and p value less than or equal to 0.05 following interaction of SRL at all-time points. Pathway analysis using GeneSpring 12.6.1 revealed that the MAPK signaling pathway is affected as early as 2h while cell cycle, DNA replication and apoptosis pathways are most significantly affected at 24 and 48h. SRL induced immediate over-expression of the transcription factor c-Jun as early as 2h. Very few miRNAs were found to be differentially regulated at 2 and 4h, however, a significant change in differentially expressed miRNAs were noticed at 12h with the miRNA gene target list significantly overlapping with the differential gene expression. The study suggests that the interaction of SRL with HT29 cells triggers apoptosis by affecting cell cycle, DNA replication and apoptosis signaling pathways. The observed effects may be initiated by affecting MAP kinase pathway and mediated by c-JUN. These findings were further validated by qRT-PCR and western blotting. The findings will enable to exploit and develop SRL as a possible targeted drug for cancer therapeutics.

2014-10-14 | E-MTAB-3003 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data