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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAi knock down of known nonsense-mediated mRNA decay (NMD) factors in Drosophila cells

ABSTRACT: All eukaryotes studied to date have the capacity to detect and degrade mRNAs harboring premature translation termination codons (PTCs) in a process called nonsense-mediated mRNA decay (NMD) (reviewed in Wagner E & Lykke-Andersen J, 2002). This surveillance system allows the cell to prevent the expression of potentially harmful truncated proteins. trans-acting factors required for NMD in Drosophila are the proteins UPF1, UPF2, UPF3, SMG-1, SMG-5 and SMG-6. To identify mRNAs naturally regulated by NMD, we analyzed expression profiles in Drosophila cells RNAi-depleted of known NMD factors using high-density oligonucleotide arrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jan Rehwinkel

PROVIDER: E-MEXP-202 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nonsense-mediated mRNA decay factors act in concert to regulate common mRNA targets.

Rehwinkel Jan J Letunic Ivica I Raes Jeroen J Bork Peer P Izaurralde Elisa E

RNA (New York, N.Y.) 20051001 10

Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that degrades mRNAs containing nonsense codons, and regulates the expression of naturally occurring transcripts. While NMD is not essential in yeast or nematodes, UPF1, a key NMD effector, is essential in mice. Here we show that NMD components are required for cell proliferation in Drosophila. This raises the question of whether NMD effectors diverged functionally during evolution. To address this question, we examined expression profi ...[more]

PMID: 16199763

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