Transcription profiling of the Arctic springtail Megaphorura arctica after cold or salt dehydration to study insect cyroprotective dehydration

ABSTRACT: The 13,824 cDNA microarray was constructed by printing 6912 PCR-amplified cDNA clones in duplicate. These were derived from a previous EST study (Clark et al, 2007) and comprised 3840 clones from the Library D2 (fully dehydrated animals, cooled to -14°C) and 3072 clones from the Library D1 (dehydrated animals cooled to -2°C). The Stratagene SpotReport Alien Array Validation System (Stratagene, La Jolla, CA, USA) was included on the microarray. Construction and hybridization of the arrays were performed as described in Purac et al (2008) with the exception of the amino-modified primers used for the initial cDNA amplification for array printing, which in this study were: (pAL32FOR: TTCTCGGGAAGCGCG and M13 forward: GTAAAACGACGGCCAG). Hybridizations were performed using control animals in comb

ORGANISM(S): Megaphorura arctica

SUBMITTER: Michael Thorne 

PROVIDER: E-MEXP-2105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress