Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

20 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative genomic hybridization of E. faecalis ORGR1F transconjugants mated with V583 to determine the amount of DNA transfered between strains

ABSTRACT: Comparative genome hybridization of transconjugants of E. faecalis OG1RF mated with V583. The total DNA of transconjugants was compared with wildtype strains to ascertain the amount of DNA that was transferred from E. faecalis V583 to E. faecalis OG1RF.

ORGANISM(S): Enterococcus faecalis

SUBMITTER: Janet Manson

PROVIDER: E-MEXP-2135 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Comparative genomic hybridization of diverse Enterococcus faecalis strains to determine the core species genome

Project description:Comparative genomic hybridization analysis of diverse Enterococcus faecalis strains to determine core species genome.<br>An additional data file, Gilmore_M-Genomotyping_Tables_12-21-2006.xls, containing information about data processing is available for download from <a href="ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-1090/">ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-1090/</a>

2007-06-04 | E-MEXP-1090 | biostudies-arrayexpress

The SOS response controls intrinsic resistance to cephalosporins

Project description:Comparison of the transcription profile between Enterococcus faecalis V583 strain and its mutant having a constitutive SOS response

2017-07-05 | E-MEXP-3861 | biostudies-arrayexpress

Transcriptome profiling of tdc cluster delection mutant of Enterococcus faecalis V583

Project description:The aim was to study the transcriptional profiling of the tdc cluster delection mutant E. faecalis V583 Î?tdc (non-tyramine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine. E. faecalis V583 Î?tdc cells (test) compared with E. faecalis V583 cells (reference). Both strains grown in GM17 medium suplemented 15 mM tyrosine.

2016-02-15 | E-GEOD-77864 | biostudies-arrayexpress

The Enterococcus faecalis V583 transcriptional profile during survival within macrophages

Project description:Transcriptional profiling of E. faecalis V583 during intracellular survival compared to V583 grown to mid-log phase (OD 600 = 0.05) in THB + 1% glucose. In this study we report the complete intracellular E. faecalis V583 transcriptome following infection of RAW264.7 macrophages. During intracellular survival, approximately 45% of the V583 genome was differentially regulated including numerous genes involved in the oxidative stress, heat shock and SOS responses. We observed that the E. faecalis-containing phagosome was limited for glycolytic substrates, nucleotides, amino acids and numerous ions necessary for growth and protein function. Approximately 35% of the genes differentially regulated during survival within macrophages were of hypothetical/unknown function, suggesting that the V583 response to phagocytosis involves many previously unstudied loci. Here, we provide the first comprehensive study elucidating the transcriptional response of E. faecalis to phagocytosis, which may provide new targets for future studies. Bacterial RNA was obtained from RAW264.7 macrophage infected with E. faecalis V583 4, 8 and 12 h post-infection and compared to E. faecalis V583 RNA extracted from mid log growth (OD 600 = 0.05). Control cultures were grown in Todd-Hewett Broth supplemented with 1% glucose.

2011-12-21 | E-GEOD-34590 | biostudies-arrayexpress

Transcriptomics analysis of E. faecalis V583 exposed to subinhibitory concentration of chlorhexidine

Project description:The vancomycin resistant strain V583 was exposed to subinhibitory concentration of chlorhexidine. E. faecalis V583 cells were collected at 15 minutes after chlorhexidine addition and without chlorhexidine, for RNA extraction and hybridization on Affymetrix microarrays.

2017-01-11 | E-MTAB-5181 | biostudies-arrayexpress

Comparative genomic hybridization of several Enterococcus faecalis strains compared to strain V583

Project description:Gene content in various Enterococcus faecalis strains compared to E. faecalis V583. Strains have been compared to the V583 strain by comparative genomic hybridization using genome-wide PCR-based microarrays representing the V583 genome. Genes have been deemed "present" or "divergent" in the various strains.

2007-01-15 | E-MEXP-931 | biostudies-arrayexpress

E. faecalis transcriptional responses to zinc stress

Project description:Transcriptional profiling of Enterococcus faecalis V583 comparing the transcriptome of cells grown in M17 + glucose 0.5% (GM17) with the transcriptome of cells grown in M17 + glucose 0.5% + 4 mM ZnSO4 2 condition experiment: V583 grown in GM17 vs. V583 grown in GM17 + 4mM ZnSO4. Two biological replicates and a dye-swap. Three replicates per array.

2011-08-21 | E-GEOD-30947 | biostudies-arrayexpress

E. faecalis transcriptional responses to manganese stress

Project description:Transcriptional profiling of Enterococcus faecalis V583 comparing the transcriptome of cells grown in M17 + glucose 0.5% (GM17) with the transcriptome of cells grown in M17 + glucose 0.5% + 4 mM MnSO4 2 condition experiment: V583 grown in GM17 vs. V583 grown in GM17 + 0.4mM MnSO4. Three biological replicates, one of which is a dye-swap. Three replicates per array.

2011-08-21 | E-GEOD-30948 | biostudies-arrayexpress

E. faecalis transcriptional responses to copper stress

Project description:Transcriptional profiling of Enterococcus faecalis V583 comparing the transcriptome of cells grown in M17 + glucose 0.5% (GM17) with the transcriptome of cells grown in M17 + glucose 0.5% + 0.05 mM CuSO4 2 condition experiment: V583 grown in GM17 vs. V583 grown in GM17 + 0.05mM CuSO4. Three biological replicates, one of which is a dye-swap. Three replicates per array.

2011-08-21 | E-GEOD-30949 | biostudies-arrayexpress

Transcriptomic response of Enterococcus faecalis to iron excess

Project description:To further investigate the homeostatic response of E. faecalis to Fe exposure, we examine the whole-genome transcriptional response of wild-type (WT) exposed to non toxic Fe excess. This experiment correspond the work titled Transcriptomic response of Enterococcus faecalis to iron excess (work in preparation) A four chip study using total RNA recovered from four separate wild-type cultures of Enterococcus faecalis OG1RF, two controls samples (N medium growth) and two iron samples (N medium gowth with 0.5 mM Fe-NTA). Each chip measures the expression level of 3,114 genome genes from Enterococcus faecalis strain V583 (A7980-00-01).

2012-03-01 | E-GEOD-34432 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data