Transcription profiling of Pseudomonas aeruginosa representational genomic libraries to identify aminoglycoside resistance genes

ABSTRACT: For this study, we created three highly representational different sized genomic libraries of P. aeruginosa PAO1 within the vector pBTB-1. Vector pBTB-1 is a low copy number broad host range plasmid containing a ß-lactamase resistance marker, a pBAD promoter upstream of the cloning site, as well as transcriptional terminators flanking the cloning site to aid in insert stability. These libraries were transformed into the recombination-deficient P. aeruginosa PAO1 mutant, PAO2003, pooled, and parallel selections performed to identify via traditional sequencing or SCALEs the genomic regions capable of conferring increased tolerance to amikacin, gentamicin, or tobramycin. These antibiotics are all structurally related but have differing substitution patterns on their aminoglycoside backbones.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Julie Struble 

PROVIDER: E-MEXP-2396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress