Transcription profiling by array of mouse hippocampus under ibotenic acid treatment.

ABSTRACT: Mice (n=85) were anaesthetised by ip injection (0.1 ml/10 g) of xylazine (5%) and ketamine (10%) diluted in sterile water. After placing animals in a stereotactic frame, the skull surface was exposed and holes were drilled at the appropriate sites to allow bilateral infusion of ibotenic acid (10 ï¾µg/ï¾µl in saline) with a glass capillary (40 ï¾µm external tip) glued to a 1 ï¾µl Hamilton syringe connected to stereotactic syringe pump (KDS 310, KD scientific). Antero-posterior (AP), lateral (L) and vertical (V) coordinates (in mm) for micro-infusions in the dorsal hippocampus, were taken relative to the bregma: (1) AP -1.7, L +/- 1, V -1.5; (2) (2) AP -2.2, L +/- 1.5, V -1.5; (3) AP -2.6, L+/- 2, V -1.5 (based on brain atlas by Paxinos and Franklin, 2004). The infused volume of ibotenic acid or PBS was 30 nl per injection site. The rate of infusion was 30 nl/min and the needle was left in situ for another 2 min, before being slowly retracted.

ORGANISM(S): Mus musculus

SUBMITTER: Stephane Girard 

PROVIDER: E-MEXP-2682 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress