Transcription profiling by array of lymphocytes from actue lymphoblastic leukemia patients immediately after total body irradation

ABSTRACT: To identify genes and related pathways that are regulated by radiotherapy in acute lymphoblastic leukemia (ALL) patients’ lymphocytes, we have employed whole genome microarray expression profiling as a discovery platform to identify genes associated with total body irradiation (TBI) therapy. Purified peripheral lymphocytes were sampled 24 h after each 4.5 Gray TBI treatment (total 9 Gray) from 4 adult patients receiving hematopoietic stem cell transplantation, and the results showed that 478 genes were differentially expressed with increasing TBI dose, and serial test of cluster (STC) analysis suggested three major patterns in these genes. Modulated genes were constructed to co-expression network and identified significant GO as follow: ubiquitin-dependent protein catabolic process, transcription, proteolysis and apoptosis et al. The reliability of the data obtained from the microarray was verified by performing quantitative real-time PCR on 12 representative genes. This study provides candidate genes associated with TBI sensitivity for further research.

ORGANISM(S): Homo sapiens

SUBMITTER: YIde Hu 

PROVIDER: E-MEXP-2964 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress