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DNA_library hybridization

ABSTRACT: PCR read-off microarrays: The ability to efficiently and economically generate libraries of defined pieces of DNA would have a myriad of applications, not least in the area of defined or directed sequencing and synthetic biology, such as protein engineering and aptamer library screening, but also in applications associated with encoding and tagging. By this approach DNA microarrays were used to allow the linear amplification off immobilized DNA sequences on an array followed by PCR amplification. Arrays of increasing sophistication (1; 10; 3,875; 10,000 defined sequences) were used to validate the process, with sequences verified by selective hybridization to a complementary DNA microarray and DNA sequencing. This technique offers an economical and efficient way of producing hundreds to thousands of specific DNA libraries, while the DNA-arrays can be used as M-^SfactoriesM-^T allowing specific DNA oligonucleotide pools to be generated with or without masking.

ORGANISM(S): Homo sapiens

SUBMITTER: Nina Svensen

PROVIDER: E-MEXP-3099 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Decoding a PNA encoded peptide library by PCR: the discovery of new cell surface receptor ligands.

Svensen Nina N Díaz-Mochón Juan José JJ Bradley Mark M

Chemistry & biology 20111001 10

The ability to screen and identify new ligands for cell surface receptors has been a long-standing goal as it might allow targeting of pharmaceutically relevant receptors, such as integrins or G protein coupled receptors. Here, we present a method to amplify hits from a library of PNA-tagged peptides. To this end, human cells, overexpressing either integrins or the CCR6 receptor, were treated with a 10,000 member PNA-encoded peptide library. Extraction of the PNA tags from the surface of the cel ...[more]

PMID: 22035797

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