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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Met4 hyperactivation timecourse

ABSTRACT: The met31 single delete and the met32 single delete strains were analyzed for genome-wide transcription at different times following Met4 hyeractivation and compared to cells that contain both Met31 and Met32.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Traci Lee

PROVIDER: E-MEXP-3301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Characterizing the roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.

Carrillo Emilio E Ben-Ari Giora G Wildenhain Jan J Tyers Mike M Grammentz Dilon D Lee Traci A TA

Molecular biology of the cell 20120321 10

Yeast sulfur metabolism is transcriptionally regulated by the activator Met4. Met4 lacks DNA-binding ability and relies on interactions with Met31 and Met32, paralogous proteins that bind the same cis-regulatory element, to activate its targets. Although Met31 and Met32 are redundant for growth in the absence of methionine, studies indicate that Met32 has a prominent role over Met31 when Met30, a negative regulator of Met4 and Met32, is inactive. To characterize different roles of Met31 and Met3 ...[more]

PMID: 22438580

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