Effects of Carbon Nanotubes Substrates on dendritic cells

ABSTRACT: To assess the effect of carbon nanotubes substrated on dendritic cell (DCs) properties, DCs were generated from human monocytes of healthy donors as previously described (Aldinucci A et al, 2010). In particular, isolated monocytes were cultured for 6 days in medium supplemented with GM-CSF (1000U/ml) and IL-4 (1000U/ml), in presence or absence of multi-walled carbon nanotubes (MWCNT); then DCs were activated by 24 hours of incubation with LPS (1ug/ml). RNA extracted from floating and CNT adherent DCs were then used for transcriptional profilingthen used

ORGANISM(S): Homo sapiens

SUBMITTER: Lisa Rizzetto 

PROVIDER: E-MEXP-3560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress