Development of a Neural Teratogenicity Test Based on Human Embryonic Stem Cells- Response to Retinoic Acid Exposure

ABSTRACT: The aim of this study was the development of an alternative testing method based on human embryonic stem cells for prenatal developmental toxicity with particular emphasis on early neural development. To this purpose, we designed an in vitro protocol based on the generation of neural rosettes, representing the in vitro counterpart of the developing neural plate and neural tube, and we challenged this complex cell model with retinoic acid (RA), a well-known teratogenic agent. The cells were exposed to different concentrations of RA during the process of rosettes formation. Morphological and molecular parameters were evaluated in treated as compared with untreated cells to detect both cytotoxicity and specific neural toxicity. Transcriptomic analysis was performed with microarray Affymetrix

ORGANISM(S): Homo sapiens

SUBMITTER: John Antonydas Gaspar 

PROVIDER: E-MEXP-3577 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress