Proteomics

Dataset Information

39

Proteome of ZIKV-infected and NS4B-transduced hNPC (H/PF/2013)


ABSTRACT: To investigate the effects of ZIKV infection or ZIKV-NS4B-transduction on the global proteome scale at early stages of hNPC differentiation into neurons, hNPC cells were infected with ZIKV (Asian strain: H/PF/2013; MOI=0.01) or transduced with ZIKV-NS4B or HCV-NS4B and one day later cells were either left under proliferative conditions or neuronal differentiation was induced with ROCK inhibitors treatment and growth factors withdrawals. Five days later samples were harvested and processed for quantitative label-free proteomics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013   Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Zika Virus Congenital Syndrome

SUBMITTER: Pietro Scaturro  

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD009557 | Pride | 2018-09-03

REPOSITORIES: Pride

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Publications


Zika virus (ZIKV) has recently emerged as a global health concern owing to its widespread diffusion and its association with severe neurological symptoms and microcephaly in newborns1. However, the molecular mechanisms that are responsible for the pathogenicity of ZIKV remain largely unknown. Here we use human neural progenitor cells and the neuronal cell line SK-N-BE2 in an integrated proteomics approach to characterize the cellular responses to viral infection at the proteome and phosphoproteo  ...[more]

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