ABSTRACT: Microarrays were used to identify genes that participate in the acid responce tolerance of C. jejuni. Three biological replicates of two different conditions (pH 5.5 and 7). Two slides and every slide contained three arrays- see attached additional file.
Project description:The effect of cell free supernatant from the antifungal strain Lactobacillus plantarum 16 on the growth of Aspergillus fumigatus Af293 was assessed. Transcriptome analysis of the genome was performed after ten minutes exposure to antifungal supernatant in order to determine the molecular targets involved in inhibtion.
Project description:The experimental design was to determine the regulon of the transcriptional activator RarA. This was done by comparing the transcriptome profiles between a rarA overexpressor and the baseline control. RarA overexpression was achieved by plasmid mediated overexpression of the rarA gene and the baseline control was a vector only strain.
Project description:Expression arrays comparing Campylobacter jejuni NCTC11168 during growth in the cecum of germ-free C57 BL/6 IL-10 knockout mice to C. jejuni NCTC11168 during growth in Bolton broth. Four biological replicates comparing C. jejuni NCTC11168 growth in vivo to in vitro. Two biological replicates were dye swaps.
Project description:Methicillin-resistant Staphylococcus aureus is one of the major causative agents associated to infections with a high morbidity and mortality in hospitals worldwide. In previous studies, we reported that lignan 3'-demethoxy-6-O-demethylisoguaiacin isolated and characterized from Larrea tridentata showed the best activity towards methicillin-resistant S. aureus. Understanding of mechanism of action of drugs allows design drugs in a better way. Therefore, we employed microarray to obtain gene expression profile of methicillin-resistant S. aureus after exposure to 3'-demethoxy-6-O-demethylisoguaiacin. The results showed that lignan had an effect on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This study consisted of comparison of isolated RNA of MRSA not treated and MRSA treated with lignan 3'-demethoxy-6-O-demethylisoguaiacin. Both RNAs samples were differentially dyed with Cy3 and Cy5 during cDNA synthesis and hybridized on DNA chip. Afterwards, the chip was scanned in a GenePix 4000B scanner. The resulting gene expression profile was analyzed in databases for functional annotations to find a potential mechanism of the lignan in MRSA.
Project description:rat PC-12 cells were infected with a lentivirus expressing a sponge cassette to knockdown the expression of miR-132. Agilent whole rat genome arrays were probed to screen for changes in miR-132 knockdown cells versus control infected cells.
Project description:High-resoution array comparative genomic hybridization of pulmonary carcinoids with a favorable and a poor disease outcome. Data set from a complementary experiment looking for differentially expressed genes is also deposited at ArrayExpress under accession ID E-MEXP-3790 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-3790).