ABSTRACT: We characterized the global microRNA expression of five commonly used hematopoietic cell lines: Kasumi-1, REH, U-937, BV-173 and ME-1. RNA samples from all five cell lines were compared to reference material composed of RNA from 20 different tissues
Project description:Expression profiling of micro RNAs in mouse embryonic gonads before (11.5 days post coitum) and after (13.5 days post coitum) the critical period of sex determination
Project description:To gain insight into miRNAs involved in the regulation of the human innate immune response, we screened for differentially expressed miRNAs in circulating leukocytes in an in vivo model of acute inflammation triggered by E. coli lipopolysaccharide (LPS) infusion.<br>Leukocyte RNA was isolated from venous blood samples obtained from healthy male volunteers before and 4 hours after LPS-infusion. After fluorescence labeling, RNA samples were hybridized to microarrays containing capture probes for measuring the abundance<br>of more than 600 human miRNAs (Exiqon).
Project description:Parallel transcription profiling of cardiomyocyte clusters derived from human embryonic stem cells. Both mRNA (E-MEXP-2654) and miRNA (this experiment) transcription are measured and fetal and adult heart tissues are included as controls. This experiment was updated on 7th July 2011 to fix incorrect AH6 and FH3 files
Project description:We characterized the genomes of five commonly used hematopoietic cell lines: Kasumi-1, REH, U-937, BV-173 and ME-1. DNA samples from all five cell lines were compared to commercial sex-matched DNA.
Project description:Microarrays displaying zebrafish microRNA specific probes were hybridized using RNA samples originating from livers of male zebrafish exposed to 17-beta estradiol (E2) for 0, 24 and 48 hours.
Project description:H69 cells (human cholangiocytes) were exposed to TNF-alpha (10 ng/ml) for 8 h and were then collected for miRNA analysis, compared with non-treated control cells.
Project description:H69 cells were cultured in H69 medium with 1 ng/ml lipopolysaccharide(LPS, for smaples 04, 05 and 06) or without LPS(for samples 01, 02 and 03) for 8 hours and then collected for array analysis. <br>