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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis Potyvirus turnip mosaic virus infection stages

ABSTRACT: A model system of Potyvirus turnip mosaic virus and Arabidopsis was used in this experiment. GFP-tagged virus supplied a visualized marker for us to localize the viral infection foci and its expansion on leaf under UV light. Initially, we dissect an individual infection focus and its adjacent region into four parts and define those four parts as zone 0, 1, 2, and 3, which represented different viral infection stages respectively. Corresponding fours parts were also dissected from control plant treated with turnip leaf sap only. This process was replicated three times totally.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Chunling Yang

PROVIDER: E-MEXP-509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Spatial analysis of arabidopsis thaliana gene expression in response to Turnip mosaic virus infection.

Yang Chunling C Guo Rong R Jie Fei F Nettleton Dan D Peng Jiqing J Carr Tyrell T Yeakley Joanne M JM Fan Jian-Bing JB Whitham Steven A SA

Molecular plant-microbe interactions : MPMI 20070401 4

Virus-infected leaf tissues comprise a heterogeneous mixture of cells at different stages of infection. The spatial and temporal relationships between sites of virus accumulation and the accompanying host responses, such as altered host gene expression, are not well defined. To address this issue, we utilized Turnip mosaic virus (TuMV) tagged with the green fluorescent protein to guide the dissection of infection foci into four distinct zones. The abundance of Arabidopsis thaliana mRNA transcrip ...[more]

PMID: 17427806

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