Transcription profiling of CACNA1E overexpression in human embryonic kidney HEK293 cells to investigate the involvement of CACNA1E in Wilms tumorigenesis

ABSTRACT: Wilms tumour (nephroblastoma) is the most common paediatric renal tumour, affecting 1 in 10,000 children. Despite the excellent survival of patients treated with a combination of surgery, chemotherapy and radiotherapy, approximately 15% will relapse and die of this disease. We have identified CACNA1E to be significantly overexpressed in relapsing tumours compared to non-relapsing. To investigate the involvement of CACNA1E in Wilms tumorigenesis, CACNA1E stably transfected HEK293 cell line and parental lines were obtained, grown and RNA extracted. To assess differential gene expression between the two cell lines, RNA was hybridised to Affymetrix Human Genome U133 Plus 2.0 GeneChip. Human embryonic kidney HEK293 parental and HEK2C6, (CACNA1E stably transfected cells) were kindly provided by

ORGANISM(S): Homo sapiens

SUBMITTER: Gordan Vujanic 

PROVIDER: E-MEXP-587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress