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Transcription profiling of mouse primary dermal fibroblasts treated with endothelin-1

ABSTRACT: Mouse primary dermal fibroblasts were treated with 100 nM endothelin-1 (ET1) synthetic peptide for 24 hours. Control samples received no ET1 peptide. The experiment compared treated to untreated to identify gene expression changes due to ET1 exposure. There are three biological replicates for both control and treated samples. These biological replicates represent separate derivations of primary dermal fibroblasts from genetically identical mouse litters aged 0-3 days.

ORGANISM(S): Mus musculus

SUBMITTER: Tammy Vallender

PROVIDER: E-MEXP-700 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Localized methylation in the key regulator gene endothelin-1 is associated with cell type-specific transcriptional silencing.

Vallender Tammy W TW Lahn Bruce T BT

FEBS letters 20060714 18

DNA methylation can contribute to the stable transcriptional silencing of mammalian genes. Often times, these genes are important developmental regulators, and their silencing in cell types where they are not supposed to be active is important for the phenotypic stability of the cells. To identify key developmental regulator genes whose expression in terminally differentiated cells may be inhibited by DNA methylation, mouse dermal fibroblasts were demethylated with 5-aza-2'-deoxycytidine, and ch ...[more]

PMID: 16870175

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Transcription profiling of mouse dermal fibroblasts following exposure to endothelin-1

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