Transcription profiling of E. coli strains MG Kan N15(+), MG Kan-Tos N15(-) and MG Kan-Tos N15(+) which has a linear genome made by MG Kan N15(+) and MG Kan-Tos N15(-)
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ABSTRACT: A comparison of transcriptional profiles between MG Kan N15 (+) or MG Kan-Tos N15 (-), which are reference strains and MG Kan-Tos N15 (+) strain. The strain, MG Kan-Tos N15 (+), has linear genome which are made by MG Kan N15 (+) and MG Kan-Tos N15 (-).
Project description:E. coli TG1 with pBS(Kan)Synhox can produce more hydrogen than TG1/pBS(Kan). To reveal the difference of metabolic activity (gene expression) between these strains in producing hydrogen, the differential gene expression analyses were performed. All samples cultured in complex medium with fructose containg 5 mM IPTG. Keywords: hydrogen production
Project description:E. coli RNA was hybridized to tiling arrays to study the pattern of gene expression across the chromosome. 1 sample for stationary phase and 1 for mid-exponential phase
Project description:E. coli TG1 with pBS(Kan)Synhox can produce more hydrogen than TG1/pBS(Kan). To reveal the difference of metabolic activity (gene expression) between these strains in producing hydrogen, the differential gene expression analyses were performed. All samples cultured in complex medium with fructose containg 5 mM IPTG. Experiment Overall Design: Strains: E. coli TG1/pBS(Kan)Synhox and TG1/pBS(Kan) Experiment Overall Design: Medium: Complex with fructose Experiment Overall Design: Hydrogen producing cells Experiment Overall Design: Time: 6 hour
Project description:All yeast strains were in the genetic background W303-1A (MATa leu2-3,112 trp1-1 ura3-1 can1-100 ade2-1 his3-11,15). Gene expression profiles of the following mutant strains were compared with WT: YIA29 (mdl1::HIS3); YIA30 (yme1::KAN); YIA31 (mdl1::HIS3 yme1::KAN); 2 independent RNA preparations per strain were used (A and B); 2 independent array hybridisations per RNA preparation were performed (color switch experiments).
Project description:Investigation of whole genome gene expression level changes in a Nitrosomonas europaea (ATCC 19718) wildtype and pFur::Kan mutant [kanamycin resistance cassette insertion in the promoter region of the fur gene (NE0616)] strains grown in Fe-replete and Fe-limited media. The Nitrosomonas europaea (ATCC 19718) wiltype cells grown in Fe-limited media were compared to cells grown in Fe-replete media to gain a better understanding of the metabolic changes occurring in response to iron stress. The Nitrosomonas europaea (ATCC 19718) pFur::Kan mutant strain grown in Fe-replete & Fe-limited media were compared to wildtype cells grown in Fe=replete & Fe-limited media to gain a better understanding of the role Fur (NE0616) plays in iron homeostasis control.