Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling and cross species hybridization of Sordaria macrosopora samples undergoing sexual development (wt.sex), wild type growing vegetatively (wt.veg), and mutant strains pro1, pro41, and pro1/41 on Neurospora crassa arrays

ABSTRACT: Cross-species hybridizations of Sordaria macrospora targets on Neurospora crassa microarrays were performed with targets derived from the S. macrospora wild type undergoing sexual development (wt.sex), the wild type growing vegetatively (wt.veg), and the mutant strains pro1, pro41, and pro1/41 (pro1.41). For each strain or growth condition, two independent experiments were carried out with a dye switch in the second experiment.
The following slide/target/dye combinations were done:
slide N12-105: Cy3-target: wt.veg (1), Cy5-target: pro1.41 (2)
slide N12-106: Cy3-target: wt.sex (1), Cy5-target: wt.veg(2)
slide N12-108: Cy3-target: pro1 (1), Cy5-target: wt.sex(2)
slide N12-109: Cy3-target: pro41(1), Cy5-target: pro1 (2)
slide N12-110: Cy3-target: pro1.41(1), Cy5-target: pro41 (2)

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Sordaria macrospora

SUBMITTER: Minou Nowrousian

PROVIDER: E-MEXP-915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora.

Nowrousian Minou M Frank Sandra S Koers Sandra S Strauch Peter P Weitner Thomas T Ringelberg Carol C Dunlap Jay C JC Loros Jennifer J JJ Kück Ulrich U

Molecular microbiology 20070501 4

The filamentous fungus Sordaria macrospora develops complex fruiting bodies (perithecia) to propagate its sexual spores. Here, we present an analysis of the sterile mutant pro41 that is unable to produce mature fruiting bodies. The mutant carries a deletion of 4 kb and is complemented by the pro41 open reading frame that is contained within the region deleted in the mutant. In silico analyses predict PRO41 to be an endoplasmic reticulum (ER) membrane protein, and a PRO41-EGFP fusion protein colo ...[more]

PMID: 17501918

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Dataset Information

Transcription profiling and cross species hybridization of Sordaria macrosopora samples undergoing sexual development (wt.sex), wild type growing vegetatively (wt.veg), and mutant strains pro1, pro41, and pro1/41 on Neurospora crassa arrays

Publications

The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora.

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