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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Burkholderia cenocepacia J2315 exposed to the aminothiol cysteamine during growth in synthetic cystic fibrosis sputum media (SCFM2)

ABSTRACT: Derived from coenzyme A degradation in mammalian cells, the aminothiol cysteamine may represent a promising new adjunct treatment for pulmonary exacerbations in cystic fibrosis (CF). This experiment aims to characterise the effect of sub-MIC cysteamine on Burkholderia cenocepacia J2315. Samples were cultured in SCFM2 (triplicate), and exposed to either cysteamine or a H2O control.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Burkholderia cenocepacia

SUBMITTER: Stephen Dolan

PROVIDER: E-MTAB-10327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pseudomonas aeruginosa PA14 exposed to the aminothiol cysteamine during growth in synthetic cystic fibrosis sputum media (SCFM2)

Project description:Derived from coenzyme A degradation in mammalian cells, the aminothiol cysteamine may represent a promising new adjunct treatment for pulmonary exacerbations in cystic fibrosis (CF). This experiment aims to characterise the effect of sub-MIC cysteamine on P. aeruginosa strain PA14. Samples were cultured in SCFM2 (triplicate), and exposed to either cysteamine or a H2O control.

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Project description:Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain. For expression profiling, over-night cultures of the Burkholderia cenocepacia J2315 wild-type strain and the isogenic mutants hfq::Tp and M-NM-^Thfq2 grown in LB medium were diluted to an initial OD640 nm of 0.25 into LB medium. Triplicate samples were cultured at 37M-BM-:C with 250 r.p.m. agitation for 16 h and RNA extracted from the three bacterial isolates.

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Project description:Burkholderia cenocepacia J2315 exposed to the aminothiol cysteamine during growth in synthetic cystic fibrosis sputum media (SCFM2)

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Burkholderia cenocepacia J2315

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Cysteamine inhibits glycine utilisation and disrupts virulence in Pseudomonas aeruginosa

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Altered gene expression in blood and sputum from COPD patients with frequent exacerbations

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