Microarray-based gene expression analysis of MDA-MB-231cells treated with platinum complex compared with untreated control

ABSTRACT: MDA-MB-231 cells were treated with a platinum complex or DMF for 96 h, after which, the total RNA was extracted from MDA-MB-231 cells using Trizol reagent (Life Technologies, Carlsbad, CA, USA) and purified with a RNeasy mini kit (Qiagen, Valencia, CA, USA). Biotinylated cDNA was prepared according to the standard Affymetrix protocol from 50 ng total RNA by using Ambion® WT Expression Kit. Following labeling, fragmented cDNA was hybridized for 16 h at 45 °C on Clariom ™ D Assay (Human, Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450. All arrays were scanned by using Affymetrix® GeneChip Command Console (AGCC) which was installed in GeneChip® Scanner 3000 7G. The row data(.cel) were normalized by the software Transcriptome Analysis Console (TAC; Vension

ORGANISM(S): Homo sapiens

SUBMITTER: Jingjing Zhang 

PROVIDER: E-MTAB-10554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress