Transcriptomic profiling of insecticide resistant field Anopheles funestus from Tanzania compared to a fully susceptible laboratory strain
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ABSTRACT: Comparative gene expression profiling of Anopheles funestus insecticide resistant field strain from an area of a major insecticide treated bednet randomised control trial
Project description:Comparative gene expression profiling of Anopheles gambiae insecticide resistant field strain from an area of a major insecticide treated bednet randomised control trial
Project description:Transcriptome profiling of insecticide resistant field populations of Anopheles funestus from Benin compared to a susceptible lab strain FANG
Project description:Resistance to pyrethroids, the only insecticide approved for bednets, threatens control of the major malaria vector, Anopheles funestus, in Malawi. To improve the management of such resistance countrywide, it is crucial to understand the dynamics and mechanisms driving resistance in the field. In this study the levels of insecticide resistance were determined across the highly endemic densely populated lake and southern agricultural area. Insecticide resistance to pyrethoids was assessed using standardized WHO bioassay methods and resistant mosquitoes were hybridized to susceptible mosquitoes. This microarray analysis revealed the key role of cytochrome P450 genes such as CYP6P9a, CYP6P9b and CYP6M7. However, a significant shift in the over-expression of these CYP450s was detected across a south/north transect, with CYP6M7 more highly over-transcribed in the two northern collection sites and the tandemly duplicated genes, CYP6P9a and CYP6P9b, more greatly over-transcribed in the south.
Project description:Nine Anopheles gambiae populations were sampled in three areas of Tanzania showing contrasting agriculture activity, urbanization and usage of insecticides for vector control. Insecticide resistance levels were measured in larvae and adults through bioassays with deltamethrin, DDT and bendiocarb. A microarray approach was used for identifying transcription level variations associated to different environments and insecticide resistance. the Ifakara strain originating from central Tanzania and susceptible to all insecticides was used as a reference strain.
Project description:We report the use of RNA-seq data to assemble transcriptional units of adult Anopheles funestus female mosquitoes. We also analyzed expression levels and protein divergence and discovered SNPs.
Project description:Transcripcional responses in three genotypes of Muzus persicae, each exhibiting different resistance mechanisms, in response to an anti.cholinesterase insecticide Two-condition experiment in threee different genotypes: Insecticide vs. acetone plus water. Genotypes S (exhibiting no resistance mechanism) Biological replicates: 3. Genotypes RS (resistant through a kdr sodium channel mutation) Biological replicates: 2. Genotypes MR (carrying kdr and MACE mutations) Biological replicates: 2.
Project description:Transcripcional responses in three genotypes of Muzus persicae, each exhibiting different resistance mechanisms, in response to an anti.cholinesterase insecticide