Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of Hml-SwitchGal4 induced pathway activation in Drosophila plasmatocytes

ABSTRACT: Drosophila carrying Hml-SwitchGal4 were crossed to different uas-constructs (uas-eGFP, uas-PGRP-LC, uas-hop, uas-tkvQD). 3rd instar larvae were either kept as control or SwitchGal4 was activated by hormone feeding for 24h. Afterwards plasmatocytes were extracted and subjected to RNA-seq.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Robert Streeck

PROVIDER: E-MTAB-10764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of resting plasmatocytes from Drosophila melanogaster third instar larvae

Project description:Resting plasmatocytes were isolated from 3rd instar larvae and subjected to RNA-seq as comparison to ChIP-seq data

2022-05-31 | E-MTAB-10759 | biostudies-arrayexpress

RNA-seq of plasmatocytes after septic injury

Project description:OrR drosophila 3rd instar larvae were subjected to septic injury with a mixture of E.coli and S.aureus at 3h, 6h and 18h. Plasmatocytes were isolated afterwards and subjected to RNA-seq

2022-05-31 | E-MTAB-10761 | biostudies-arrayexpress

RNA-seq of plasmatocytes after septic injury with trxG-RNAis

Project description:Drosophila plasmatocyes expressed control or trxG RNAis under the plasmatocyte specific Hml promotor. 6h prior to plasmatocyte collection animals were split into control and septic injury groups and treated accordingly. Afterwards plasmatocytes were isolated and polyA RNA was subjected to RNA-seq.

2022-05-31 | E-MTAB-10780 | biostudies-arrayexpress

RNA-seq of Drosophila plasmatocyte mosaics which lack PRC2/H3K27me3

Project description:Genetic mosaics were induced in 3rd instar larvae by heatshock to generate H3K27R mutants, Su(z)12 mutants or E(z) mutants

2022-05-31 | E-MTAB-10762 | biostudies-arrayexpress

ChIP-seq of Drosophila melanogaster OregonR plasmatocytes at 6h post septic injury

Project description:Drosophila 3rd instar larvae were subjected to septic injury. After 6h plasmatocytes were isolated, fixed and subjected to ChIP-seq.

2022-05-31 | E-MTAB-10760 | biostudies-arrayexpress

ChIP-seq of Drosophila melanogaster plasmatocytes against various histone modifications

Project description:To identify the presence of different chromatin states in homogeneous primary cells, Drosophila melanogaster plasmatocytes were isolated from 3rd instar larvae and subjected to cross-linked ChIP-seq using antibodies against a range of H3 histone modifications and PolII.

2022-05-31 | E-MTAB-10707 | biostudies-arrayexpress

RNA-seq of haemoless drosophila larvae compared to matching control animals

Project description:Drosophila melanogaster 3rd instar wandering larvae with a genetic construct for hemocyte ablation were collected along with genetically matched non-ablated animals, RNA was extracted from whole animals and used for RNA-seq.

2022-09-14 | E-MTAB-11095 | biostudies-arrayexpress

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (tiling array)

Project description:The miRNA 310-311-312-313 cluster from D. melanogaster (referred to as Dm310s) and the homolog from D. pseudoobscura (Dp310s) were used to transform D. melanogaster. The over-expression of UAS-miR310s in two Dm310s transgenic lines (M1-7 and M1-3) and Dp310s transgenic line P4-18 was driven by GAL4 line NP5941 with native miR-310 expression pattern. We used Drosophila Tiling 1.0 F arrays to assess the effects of miR310s over-expression on the whole transcriptome. We compared the transcriptional profiling of Dm310s and Dp310s overexpression in D.melanogaster using double-stranded cDNA followed by bioprime random labeling, and hybridization to Affy Drosophila tiling 1.0 F array. Third-instar progeny larvae from the crosses with maternal NP5941 and paternal UAS-miR-310 cluster transgenic lines were collected and third-instar larvae from the cross with maternal NP5941 and paternal w1118 were used as control. Three biological repeats were used for each stock. A processed data matrix reporting values (log2 intensity after quantile normalization) for all of the Samples is linked below as a supplementary file.

2010-06-24 | E-GEOD-15850 | biostudies-arrayexpress

Expression profiling of over-expression of conspecific and heterspecific miR-310 cluster in Drosophila (cDNA array)

2010-06-25 | E-GEOD-15847 | biostudies-arrayexpress

Drosophila CNS glial microarray

Project description:Gliogenesis in the Drosophila CNS occurs during embryogenesis and also during the postembryonic larval stages. Several glial subtypes are generated in the postembryonic CNS through the proliferation of differentiated glial cells. The genes and molecular pathways that regulate glial proliferation in the postembryonic CNS are poorly understood. In this study we aimed to use gene expressing profiling of CNS tissue enriched in glia to identify genes expressed in glial cells in the postembryonic CNS. We used microarrays to compare the gene expression profiles from the larval CNS of animals that had increased numbers of glial cells to identify genes that are expressed in glia. RNA was purified from the late third instar larval CNS from control larvae, or larvae expressing an activated form of the FGF receptor (Hlt[ACT]), or overexpressing the insulin receptor (InR) in glial cells using the glial specific driver repoGal4 to increase the number of glial cells and generate CNS tissue enriched in glia.

2013-04-24 | E-GEOD-46317 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data