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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAseq evaluation of nicotinamide seed treatment analysed in Norway spruce seedlings

ABSTRACT: Nicotinamide seed treatment is investigated as a method for production of robust plants. Previous studies have shown that nicotinamide promotes the plant defence system and decreases DNA methylation levels. The purpose of the present work was to learn more about the underlying molecular mechanisms. The RNA was extracted from roots of three months old Norway spruce seedlings treated at seed stage. Stress was an overrepresented biological process of upregulated genes. Stress response genes are involved in adaption to and defence against stress. Upregulated stress response genes in the seedling roots were for example transcription factors MYB77 and LHY and two chitinases. Chitinases defend against biotic stress, such as pine weevils. Epigenetic regulating genes were generally downregulated, such as DDM1 which is known to promote DNA-methylation. Previous works suggest the hypothesis that nicotinamide is a defense signal mediator, which is supported by the present study.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Picea abies

SUBMITTER: Anna Ohlsson

PROVIDER: E-MTAB-11007 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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