Dissecting the Mycobacterium bovis BCG response to macrophage infection to identify targets for anti-tuberculosis drug and vaccine discovery

ABSTRACT: New strategies are required to reduce the worldwide burden of tuberculosis. Intracellular survival and replication of Mycobacterium tuberculosis after macrophage phagocytosis is a fundamental step in the complex host-pathogen interactions that lead to granuloma formation and disease. Greater understanding of how the bacterium survives and thrives in these environments will inform novel drug and vaccine discovery programmes. Here, we use in-depth RNA sequencing of Mycobacterium bovis BCG from human THP-1 macrophages to describe the mycobacterial adaptations to the intracellular environment. We identify 329 significantly differentially regulated genes, highlighting cholesterol catabolism, methyl-citrate cycle and iron homeostasis as important for mycobacteria inside macrophages. Focused anal

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

SUBMITTER: Simon Waddell 

PROVIDER: E-MTAB-11107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress