Project description:To gain insight into the gene signatures directly mediated by LAP (CEBPB) for cancer cell progression, we performed RNA-seq in LAP-HepG2 versus control cells.
Project description:In this study, we used an in vitro RPE model mimicking extracellular deposit formation and atrophy to study age-related changes occurring during early-AMD pathogenesis. Analysis of gene expression profiles at 4, 12, 17, and 25 weeks of culture revealed significant alterations in the RPE transcriptome related to age. The results of this analysis, together with those obtained through other omics such as metabolomics, are relevant to improve knowledge about RPE aging and the pathophysiology of AMD.
Project description:A RPE cell model mimicking a mature RPE was used to investigate the potential anti-inflammatory effects of high-dose zinc supplementation in the context of AMD research. RPE cultures were exposed to inflammatory conditions induced by interleukin 1α (IL-1α) after a 14-day treatment with high doses of zinc. RNA sequencing was employed to examine the relationship between zinc homeostasis and transcriptional changes driven by supplementation and inflammation. By analyzing the effects of different zinc concentrations prior to IL-1α exposure, we explored the role of the Zn-metallothioneins complex in regulating inflammatory responses and cellular function. This study aims to provide valuable insights into potential therapeutic strategies for AMD that target zinc homeostasis.
Project description:To elucidate the sucrose signaling and the defense marker genes under sucrose treatment. This gene is intended for functional analysis. In addition, the underlying molecular mechanisms of this defense marker were studied. After that, investigate the underlying molecular mechanisms from sucrose treatment to pathogen defense pathway were studied.
Project description:We created a double loss-of-function/knockout mutant targeting two rice genes simultaneously. The selected genes are as follows: OsCNGC4(LOC_Os03g44440) and OsCNGC5(LOC_Os12g28260). These two CNGCs are strongly transcriptional expressed in the rice mature anthers (stages 13-14). The mutant of these OsCNGC4/5 displayed a low seed-setting rate. This data refers to the transcriptome of mature anthers from the double mutant of OsCNGC4 and OsCNGC5. We sampled mature anther for the analysis.
Project description:A RPE cell model mimicking a matured RPE was subjected to an acute inflammatory stress with and without Zn supplementation and the effects of such exposure were evaluated by studying alterations at the transcriptome level. The experiment performed aim to improve the understanding of the metabolic processes involving Zn and their relevance in the physiology and pathophysiology of neurodegenerative diseases resembling AMD disease.
Project description:Rice anthers at anthesis stage from the wild type and osrac6-1 mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.
Project description:OJAP_WD199 was annotated as partial WD40 genes, whereas showed strong pollen-specific expression. We generated CRISPR-Cas9-induced loss-of-function mutants for OJAP_WD199 and identified homozygous lines with mutations at two independent target sites per gene. Compared to wild-type (WT) plants, fertility was markedly reduced in knockout mutants, a severe reduction to around 20%. Despite these defects, morphological examination of flowers, anthers, and pollen at the pollen maturation stage revealed no obvious differences between the WT and mutant plants. For the corresponding trascriptomic analysis, we sampled mature pollen anthers from a control group and mutant group.
Project description:OJAP_WD37 was annotated as partial WD40 genes, whereas showed strong pollen-specific expression. We generated CRISPR-Cas9-induced loss-of-function mutants for OJAP_WD37 and identified homozygous lines with mutations at two independent target sites per gene. Compared to wild-type (WT) plants, fertility was markedly reduced in knockout mutants, a severe reduction to around 40%. Despite these defects, morphological examination of flowers, anthers, and pollen at the pollen maturation stage revealed no obvious differences between the WT and mutant plants. For the corresponding trascriptomic analysis, we sampled mature pollen anthers from a control group and mutant group.