Project description:In this study, we used an in vitro RPE model mimicking extracellular deposit formation and atrophy to study age-related changes occurring during early-AMD pathogenesis. Analysis of gene expression profiles at 4, 12, 17, and 25 weeks of culture revealed significant alterations in the RPE transcriptome related to age. The results of this analysis, together with those obtained through other omics such as metabolomics, are relevant to improve knowledge about RPE aging and the pathophysiology of AMD.
Project description:To elucidate the sucrose signaling and the defense marker genes under sucrose treatment. This gene is intended for functional analysis. In addition, the underlying molecular mechanisms of this defense marker were studied. After that, investigate the underlying molecular mechanisms from sucrose treatment to pathogen defense pathway were studied.
Project description:We created a double loss-of-function/knockout mutant targeting two rice genes simultaneously. The selected genes are as follows: OsCNGC4(LOC_Os03g44440) and OsCNGC5(LOC_Os12g28260). These two CNGCs are strongly transcriptional expressed in the rice mature anthers (stages 13-14). The mutant of these OsCNGC4/5 displayed a low seed-setting rate. This data refers to the transcriptome of mature anthers from the double mutant of OsCNGC4 and OsCNGC5. We sampled mature anther for the analysis.
Project description:A RPE cell model mimicking a matured RPE was subjected to an acute inflammatory stress with and without Zn supplementation and the effects of such exposure were evaluated by studying alterations at the transcriptome level. The experiment performed aim to improve the understanding of the metabolic processes involving Zn and their relevance in the physiology and pathophysiology of neurodegenerative diseases resembling AMD disease.
Project description:Rice anthers at anthesis stage from the wild type and osrac6-1 mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.
Project description:Pollen tube growth is essential for successful fertilization and stable crop yields. We constructed loss-of-function/knock-out mutants that simultaneously target two rice genes using the CRISPR/Cas9 mutagenesis system. The selected OsRALF17 and OsRALF19 genes are strongly expressed in rice bicellular/tricellular pollen and have essential functions in the pollen tube growth. For the corresponding transcriptomic analysis, we sampled mature pollen anthers from a control group and an OsRALF17/19 knock-out mutant.
Project description:We generated single loss-of-function knockout mutants targeting a specific rice gene, Ruptured Pollen Tube (RUPO, GTrD2; LOC_Os06g03610), a member of the Catharanthus roseus RLK1-like (CrRLK1L) subfamily. RUPO is strongly expressed in mature anthers and pollen, and its knockout results in reduced pollen ROS levels and impaired pollen tube elongation, leading to germination defects. Consequently, RUPO mutants fail to produce self-fertilized seeds. This dataset comprises mature anther transcriptome data from RUPO single mutants, with samples collected from mature anthers for analysis.
Project description:We generated single loss-of-function knockout mutant targeting a specific rice gene, GTrD5; LOC_Os11g20384), a member of the Suppressor of Actin 1 (Sac1) domain phosphoinositide phosphatase subfamily. GTrD5 is strongly expressed in mature anthers and pollen, and its knockout results in germination defects. Consequently, GTrD5 mutants have reduced self-fertilized seed production. This dataset comprises mature anther transcriptome data from GTrD5 single mutants, with samples collected from mature anthers for analysis.
Project description:We created a double loss-of-function/knockout mutant targeting three rice genes simultaneously. The two selected genes are as follows: OsABCG16 (LOC_Os06g51460), OsABCG28 (LOC_Os11g22350). These two ABCGs are strongly transcriptional expressed in the rice mature anthers (stages 13) and bi-/tricelluler pollen. The double mutant of these OsABCGs does not produce self-fertilizing seeds due to the short length of the pollen tube inside the pistil (male-sterile). This data is about mature anther transcriptome data about the double mutant of OsABCGs. We sampled mature anther for the analysis.
Project description:We created a triple loss-of-function/knockout mutant targeting three rice genes simultaneously. The three selected genes are as follows: OsADF1 (LOC_Os02g44470), OsADF6 (LOC_Os04g46910), and OsADF9 (LOC_Os07g30090). These three ADFs are strongly transcriptional expressed in the rice mature anthers (stages 13) and bi-/tricelluler pollen. The triple mutant of these OsADFs does not produce self-fertilizing seeds due to the short length of the pollen tube (male-sterile). This data is about mature anther transcriptome data about the triple mutant of OsADFs (ADFmT). We sampled mature anther for the analysis.