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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Up- and downregulated genes in GLTP knock-out HeLa cells compared to wildtype HeLa cells.

ABSTRACT: The total HeLa cell transcriptome analysis was carried out using Illumina HumanHT-12 v4 Expression BeadChip array. RNA was isolated from cultures of wild-type (Cas9 GLTP control) and three different GLTP gene KO clones. 100 ng of total RNA was primed using oligo-dT(T7) primers and converted to cDNA with Invitrogen SuperScript III Reverse Transcriptase. The double-stranded cDNA template was amplified and labeled with in vitro transcription to generate multiple copies of biotinylated cRNA. Purified biotinylated cRNA was hybridized to HumanHT-12 v4 expression beadchips for 18 h at 58 °C using a rocking platform at speed 5. Beadchips were washed, blocked, stained with streptavidin-Cy3, and scanned with an Illumina iScan scanner, using the manufacturer’s recommended protocols. The raw data were extracted using Illumina’s GenomeStudio software.

INSTRUMENT(S): DE13701120, Agilent 2100 bioanalyzer

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Mattjus

PROVIDER: E-MTAB-12118 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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