Whole genome sequencing of tebuconazole adaptors in Cryptococcus neoformans
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ABSTRACT: Cryptococcus neoformans lab strain H99 was spread on YPD plates supplementded with 1.5ug/ml and 2ug/ml of tebuconazole. Randomly 30 (T364-T393) and 29 (T394-T422) adaptors were chosen from 1.5ug/ml and 2ug/ml drug plate, respectively, All the 59 adaptors as well as the parent H99 were sequenced.
INSTRUMENT(S): BGISEQ-500
ORGANISM(S): Cryptococcus neoformans var. grubii H99
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 1.5ug/ml pyraclostrobin. Randomly 23 adaptors were chosen. These adaptors were sequenced.
Project description:H99 derived 2 ug/mL uniconazole adaptors: Approximately 1 million cells of Crypotoccus neoformans lab strain H99 were spread on YPD-agar plate supplemented with 2 ug/mL uniconazole. After 5 days incubation of the plate at 30℃, randomly 30 adaptors were chosen. 21 adaptors were resistant and were sequenced. H99 derived 0.25 ug/mL uniconazole adaptors: Cells of H99 were adjusted to 2500 cells/mL. The culture was supplemented with 0.25 ug/mL uniconazole. After 72 h incubation, the culture was washed, diluted and approximately 200 cells were spread on YPD-agar plate. Randomly 95 colonies were tested for resistance to uniconazole. One colony (TJ3832) was resistant and was sequenced.
Project description:In this study, H99 is a wild type Cryptococcus neoformans strain. TJ1854 is a chromosome 4 disomy strain derived from H99. Approximately 1 million cells of TJ1854 were spread on YPD plate supplemented with 6ug/ml amphtericin B. Randomlyh 27 adaptors (TJ2487-TJ2513) were chosen. These adaptors were sequenced.
Project description:In Cryptococcus neoformans, H99 is the wild type. TJ1854 is the chromosome 4 disomy strain dervied from H99. In this study, approximately 1 million cells of TJ1854 were spread on YPD plate supplemented with 32ug/ml fluconazole. Randomly 27 adaptors (TJ3249-TJ3275) were chosen. These adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plates supplemented with 32ug/ml and 64ug/ml iprodione. Randomly 29 (T2590-T2618( and 30 (T2619-2648) adaptors from 32ug/ml and 64ug/ml drug plate, respectively, were sequenced.
Project description:In Cryptococcus neoformans, H99 is the wild type. TJ1843 is a strains with segmental disomy of chromosome 11, which is derived from H99. In this study, approximately 1 million cells of TJ1843 were spread on YPD plate supplemented with 32 ug/ml fluconazole. Randomly 27 adaptors (TJ3305 and TJ3331) were chosen. These adaptors were sequenced.
Project description:In this project, H99 is the wild type Cryptococcus neoformans strain. TJ1843 is a strain with segmental disomy of chromosome 11. TJ1843 is a derivative of H99. Approximately 1 million cells of TJ1843 were spread on YPD plate supplemented with 8 ug/ml amphotericin B. Randomly 27 adaptors (TJ3409-TJ3435) were chosen. These adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 4ug/ml benomyl. Randomly 60 adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 2.5ug/ml chlorothalonil. Randomly 72 adaptors were sequenced.