RNA-seq of Pseudomonas putida KT2440 and the derived strain P. putida pig21 treated with 1-octanol and PQS (Pseudomonas quinolone signal), causing chemical membrane stress.

ABSTRACT: Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Here, we explored membrane adaptations of Pseudomonas putida KT2440, in particular outer membrane vesicle (OMV) formation in response to 1-octanol, PQS and prodigiosin, causing chemical membrane stress via RNA-seq of mRNA. Pseudomonas putida wild type KT2440 (Nelson et al. 2002) and the derived strains P. putida pig21 were cultivated in biological triplicates under continuous shaking (130 rpm) at 30 °C in 10 mL LB (lysogeny broth) medium (10 g L-1 tryptone, 5 g L-1 yeast extract, 10 g L-1 sodium chloride; Carl Roth®, Karlsruhe, Germany). Antibiotics were added to the culture medium when appropriate to the following final concentrations: 25 µg mL-1 kanamycin, 25

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Pseudomonas putida KT2440

SUBMITTER: Tobias Busche 

PROVIDER: E-MTAB-12470 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress